Sagecreation minichemi
WebDec 15, 2024 · All antibodies came from Cell Signaling Technology. Detection was performed with ECL substrate (Thermo Scientific, cat. # 32109). Digital images were taken by a MiniChemi from SAGECREATION, Beijing, China. Statistical analysis. Statistical analysis was conducted by using GraphPad Prism (Version 6.0). Data represent the mean ± SEM. WebMar 2, 2024 · After washing the membranes with PBS, the secondary antibody (goat anti-rabbit IgG(H + L) HRP, 1:1000, Affinity Biosciences) was added and incubated at 37 °C. …
Sagecreation minichemi
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WebDeer antlers are the only known mammalian organ with vascularized cartilage that can completely regenerate. Antlers are of real significance as a model of mammalian stem cell-based regeneration with particular relevance to the fields of WebMiniChemi™是由北京赛智创业研发生产的一款超小型的专门针对化学发光样品拍摄的高级成像系统.采用大尺寸、高像素、深冷CCD相机和大光圈定焦镜头的完美组合,可快速检测到极微弱的化学发光信号.
Webthe Minichemi ™ Chemiluminescence Imaging System (Sagecreation, BJ, China). Western blotting Western blotting was conducted as previously described [9]. e antibodies … WebMiniChemi™ 610 Mini Chemiluminescent Imaging and Analysis System Easy to use, compact, dedicated Western Blot imager with superior sensitivity High Sensitivity Imaging …
Webtive second antibody at room temperature. Signals were detected using MiniChemi Imager (SageCreation, Beijing, China). β-actin was used as the endogenous control. TP determination Cellular ATP levels were determined by ATP kit following the manufacturer’s protocol (Beyotime, China). In brief, WebBio-Equip
WebMay 17, 2024 · After washing each membrane with TBST (Applygen, Beijing, China) several times, Immobilon Western Chemiluminescent HRP substrate (Millipore, MA, USA) was added, and the blots were analyzed using a MiniChemi chemiluminescence 610 imaging system (Sage Creation, Beijing, China). Protein expression was normalized to β-actin levels.
WebAug 15, 2024 · To gain structural insight into Sarbecovirus ORF6-mediated nuclear transport inhibition and innate immunity suppression, we sought to determine the crystal structure … lambang kesatuan tniWebDec 23, 2024 · After extensively washed in TBST, protein bands were visualized using enhanced chemiluminescence (Sage Creation, SmartChemi ECL) and images captured by MiniChemi (Sagecreation) and analysed with ImageJ. GAPDH was used as loading control. 2.5 Calcium imaging jerk graphWebthe Minichemi ™ Chemiluminescence Imaging System (Sagecreation, BJ, China). Western blotting Western blotting was conducted as previously described [9]. e antibodies (Supplementary Table S3) used were anti-GAPDH(pAb AP0063), NAP1L1(mAb ab178687), hepatoma-derived growth factor (HDGF)(pAb 11,344– 1-AP), jun proto-oncogene (c … jerk ice creamWeb凝胶成像_凝胶成像分析系统_化学发光成像仪 -北京赛智创业科技有限公司. 中国生理学会干细胞生理专业委员会第一届学术会议. 相聚南昌-中国植物生理与植物分子生物学学会第十二 … lambang kesehatan indonesiaWebthe MiniChemi™ System (SageCreation, Beijing, China). 2.6ELISA Frozen small intestinal tissue samples (n=6/group) were thawed, homogenized over ice in PBS, centrifuged and … jerk goat curryWebMar 1, 2024 · After being sealed with 5% skim milk powder for 1 h, the antibody of the target protein was added and incubated at 4 °C overnight. Finally, after cleaning the PVDF membrane, the second antibody was incubated for 1 h. A Chemiluminescence imaging analysis system was used for protein detection (MiniChemi 610 plus, Sagecreation, China). lambang kesehatanWebAug 24, 2024 · (Sagecreation, Beijing, China). The picture showed that the DNA molecular weight of WT and Glrx2 / mice were in the range of 300–400 bp and 400–500 bp, respectively (Figure S1A). This could be explained by the construction procedure of Glrx2 / mice. A neomycin-resistance gene with upstream lambang kesdm