Mcherry parkin
Web3 jul. 2014 · Parkin becomes localized to depolarized mitochondria in a PINK1-dependent manner and polyubiquitinates multiple mitochondrial outer membrane proteins. This permits ubiquitin-binding proteins (e.g., p62 and NBR1) to target impaired mitochondria to autophagosomes via Atg8/LC3II. WebIntroduction. Two genes mutated in autosomal recessive forms of familial Parkinson disease, PINK1 and PARKIN have been suggested to play a role in mitochondrial quality …
Mcherry parkin
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WebStress-induced mitophagy, a tightly regulated process that targets dysfunctional mitochondria for autophagy-dependent degradation, mainly relies on two proteins, PINK1 and Parkin, which genes are mutated in some forms of familiar Parkinson’s Disease (PD). WebParkin promotes mitophagy but not autophagy induction. A, HeLa cells were transfected with mCherry-Parkin for 24 h and then treated with CCCP (30 M) for 6 h.
Webexpressing mCherry-Parkin (red) and GFP-LC3 (green) and labeled with MitoTracker deep red (blue) after 48 h of DOX treatment. The white box denotes a mitochondrial … Web14 jan. 2024 · In a Parkin knock-down (KD) background, overexpression of mCherry-Parkin WT significantly increased the percentage of cells with OPTN-positive …
Web分子遗传学研究证明parkin基因是 常染色体 隐性遗传性少年型 帕金森综合征 (AR-JP)的致病基因,其表达产物parkin蛋白具有E3泛素-蛋白连接酶活性.Parkin蛋白可能在维持多巴胺 … WebGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统 自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点 …
WebThe indicated HCT116 cells stably expressing mCherry-Parkin and YFPRAB7A (A and B), mCherry-Parkin alone (C), 2HA-RAB7A alone (D), or mCherry-Parkin and indicated …
Web25 feb. 2014 · WT and TBC1D15−/− cells stably expressing YFP-LC3 and mCherry-Parkin were treated with valinomycin for 3 hr. Cells were stained with (A) Alexa Fluor 635 … ed pump partsWebPlasmid pBMN-mCherry-Parkin from Dr. Richard Youle's lab contains the insert mCherry-Parkin and is published in Elife (Cambridge). 2014 Feb 25;3:e01612. doi: … ed pump how to use videoWeb20 nov. 2024 · 不同于其它从维多利亚水母中分离的GFP蛋白和其它绿色荧光蛋白变体，mCherry以及其它大多数红色荧光蛋白是从珊瑚(Discosoma)中分离出来的蛋白，相对 … ed pumps for men vacuum manualWeb(G) Cells were transfected with plasmids encoding mCherry-Parkin (200 ng) and GFP-LC3 (400 ng) and treated as in (E) and (F). Cells were immunostained for TOM20 (blue). … constantsharkhttp://jmi.fudan.edu.cn/fileup/HTML/11033.htm constants gentleWeb线粒体自噬指细胞通过自噬机制选择性除去损伤或多余的线粒体。真核生物通过线粒体自噬调控线粒体质量，维持供能细胞器的功能。大量研究表明，帕金森病相关基因PINK1 … constantshadervaluesWeb3 mrt. 2012 · During a 25-min time-lapse recording of the soma, ring-like vesicular structures co-labeled with GFP-LC3 and mCherry-Parkin disappeared whereas new puncta emerged. Furthermore, a ring-like Parkin-associated mitochondrion, as opposed to the Parkin-negative mitochondria in the same image, disappeared over a 38-min time-lapse … edpuniversity saber es poder