Lysm-cre/rosa26-tdtomato
Webmicroglial (Iba1) markers, confirm efficient, widespread, but largely cell-selective cre-mediated recombination. Quantification of % recombination of hippocampal (Hip) and cerebellar (Cer) neurons, astrocytes, and microglia in Rosa26-tdTomato/Eno2-Cre, GFAP-Cre, and LysM-cre mice respectively is shown. Note that, as previously reported WebNov 1, 2012 · Insertion of tdTomato, a variant of red fluorescent protein, into the Rosa26 locus of PSCs of various rat strains allows ubiquitous expression of tdTomato. Through germline transmission of one Rosa26-tdTomato knock-in embryonic stem cell line, we also obtained tdTomato knock-in rats.
Lysm-cre/rosa26-tdtomato
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WebFeb 5, 2014 · To isolate LysM-lineage AT2 cells for expression profiling, adult (PN70) LysM-Cre > tdTomato mice were euthanized and pulmonary vasculature perfused clear (described above). WebDec 1, 2016 · We used LysM-Cre/Rosa26-tdTomato mice in which myeloid cells and their progeny permanently express the Rosa26-Tomato (red fluorescence) reporter. Confocal …
WebApr 8, 2016 · LysM-Cre induced tdTomato expression (red) in cortical neurons is also shown in commissural (B) and projection fibers (C). Boxes indicate positions of detailed images (B and C). Of note, the section is slightly tilted so that the internal capsule is only depicted on the right side. Web将IRES-CreERT2敲入到小鼠Sftpc基因3'UTR中，他莫昔芬诱导型Cre重组酶CreERT2受内源Sftpc基因启动子驱动。 ... Rosa26 tdTomato/+ mouse. Fig2. Detection of tdTomato(red) in various tissues of Sftpc CreERT2/+;Rosa26 tdTomato/+ mice. (For more detailed information please contact our technical advisor.)
WebAug 20, 2024 · We tested and confirmed that LysM-Cre and Hdc-Cre activity was specifically detected in Mac and MC, respectively, utilizing the ROSA26 tdTomato (R26-tdT) reporter system (Figures 5 D and 5E). We further validated the residual unrecombined Gata2 floxed allele in Mac and ΜC from LysM - and Hdc -Cre mice, respectively, by … WebStock No: 007909 Protocol 29436: Standard PCR Assay - Gt (ROSA)26Sor (tdTomato-WPRE) Version 2. 2 Notes Melt curve analysis is done using a Roche Light Cycler 480. …
Weba, Histological characterization of tdTomato expression in brain from LysM-Cre; Rosa26:LSL:tdTomato. tdTomato expression is seen in red, Iba1 (microglia) in green, and neurons (NeuN) in white. Upper left shows low-power image of a mid-sagittal section. Lower left shows higher power image of cortex.
WebJul 11, 2024 · The LysM receptor-like kinases (LysM-RLKs) are important to both plant defense and symbiosis. Previous studies described three clades of LysM-RLKs: LysM … country per capita covid deathsWeb图1. TdTomato的打靶策略[1] 这种鼠在正常情况下由于3×stop的存在并不能表达红色荧光，当与Cre鼠或TAM激活的CreERT2鼠交配后，产生的后代由于Cre重组酶切割loxp位点之间的序列将3×stop切除，TdTomato得到表达，就可以在Cre或CreERT2表达的组织中发出红色荧光，指示基因的位置。 country pepperWebThe Jackson Laboratory rosa26 lsl tdtomato hereafter tdt mice. Rosa26 Lsl Tdtomato Hereafter Tdt Mice, supplied by The Jackson Laboratory, used in various techniques. … brewers burgess hill telephoneWebAug 7, 2024 · a, BFM of macrophages within the synovial tissue using the macrophage markers F4/80 (left and middle; colour as indicated) and CD68 (right; green) in ColVI cre R26-tdTomato reporter mice (left ... brewers bucket hatWebTdTomato大鼠（SD- Gt (ROSA26)tm1 (CAG-LSL-Tdtomato) /Bcgen）在ROSA位点敲入了一个CAG启动子、TdTomato蛋白编码序列和WPRE序列，并在CAG启动子和TdTomato … brewers broadcast schedule 2022WebFeb 26, 2024 · LysM-Cre mice were provided by Ira Tabas (Columbia University, New York, New York, USA) . Rosa26-loxp-mTomato-stop-loxp-mGFP (Rosa26-mT/mG) reporter mice and Rosa26-loxp-stop-loxp-tdTomato (Rosa26-Ai9) reporter mice were obtained as previously described (refs. 34, 35, respectively). All utilized mice were maintained on a … country perception indexWebWe evaluated two conditional (Csf1r-Cre and LysM-Cre) and two inducible [CX 3 CR1-estrogen receptor-Cre (ERCre) and CD68-rtTA] lines. We assessed Cre activation in six resident lung myeloid populations, as well … brewers by the bay bellingham