WebStylopine synthase (CYP719A13) from Argemone mexicana produced in Sf9 insect cells is a P450 enzyme that that catalyzes a methylenedioxy bridge on the molecule (S)-cheilanthifoline to form (S)-stylopine, an essential step in the formation of benzo[c]phenanthridine and protoberberine alkaloids... From the laboratory of Toni M. … WebAug 13, 2015 · Cheilanthifoline synthase (CFS) is a plant cytochrome P450 that is 61 to 68% identical to SalSyn, exhibits high activity when expressed in yeast , and is not glycosylated in yeast despite having one N-X-T/S site …
Efficient microbial production of stylopine using a Pichia …
WebJan 23, 2007 · Heterologous expression in Saccharomyces cerevisiae showed that both CYP719A2 and CYP719A3 had stylopine synthase activity to catalyze methylenedioxy bridge-formation from cheilanthifoline to stylopine, but not cheilanthifoline synthase activity to convert scoulerine to cheilanthifoline. Functional differences and expression … WebIn enzymology, a -cheilanthifoline synthase is an enzyme that catalyzes the chemical reaction For faster navigation, this Iframe is preloading the Wikiwand page for (S) … convection based vaporization
CYP719A subfamily of cytochrome P450 oxygenases and
WebFeb 18, 2024 · For example, in A. mexicana, CYP719A13 encodes a cytochrome P450 with activity of both stylopine and canadine synthase since it can accept cheilanthifoline (IV) as well as tetrahydrocolumbamine as substrates, transforming them into stylopine and canadine, respectively, and hence participating in sanguinarine (I) and berberine synthesis . WebJan 14, 2024 · S-cheilanthifoline synthase. synthase, (S)-cheilanthifoline. CAS registry number. 138791-27-2. Keywords. Analytical Chemistry; Reaction Type; Registry … WebJan 14, 2011 · In the sanguinarine branch pathway, the CYPs cheilanthifoline synthase and stylopine synthase catalyze the formation of two methylenedioxy bridges yielding (S)-stylopine. Tetrahydroprotoberberine cis-N -methyltransferase converts ( S )-stylopine to ( S )- cis-N -methylstylopine, followed by two additional CYPs, N -methylstylopine 14 … convection baking at high altitude