WebQuantitative trait locus (QTL) mapping and bulk segregant analysis using a set of WGS data (QTL-seq) detected a major QTL on the upper arm of chromosome 3 for BPB, SB, and DTH traits within the 1.0–1.9 Mb position. Additional QTLs associated with BPB and SB were also identified from other chromosomes by the QTL-seq analysis. The QTLs ... WebJan 20, 2024 · Next Generation Sequencing Bulk Segregant Analysis (NGS-BSA) is efficient in detecting quantitative trait loci (QTL). Despite the popularity of NGS-BSA and …
GitHub - bmansfeld/QTLseqr: QTLseqr is an R package for …
WebDec 21, 2024 · INTRODUCTION. Bulk segregation analysis (BSA) was first proposed by Michelmore et al. () (Department of Vegetable Crops, University of California, Davis) and was used to identify molecular markers linked to DM5/8 (downy mildew resistance loci in lettuce) in F 2 populations.Sample pooling was performed according to the two extreme … WebSince the early 1990’s, Bulk Segregant Analysis (BSA) has been a valuable tool for rapidly identifying markers in a genomic region associated with a trait of interest (Giovannoni et … coofay backpack
Bulk segregation analysis in the NGS era: a review of its
WebNov 25, 2024 · Samples from 30 high-sensitive and 30 resistant lines were collected and bulked for each extreme for resequencing and BSA (bulk segregant analysis). Pathogenicity tests using colonized sorghum grains as inoculum White sorghum grains, soaked overnight in a 250 ml flask, were used for inoculum preparation. WebMay 31, 2024 · Next-generation sequencing (NGS)-based bulked-segregant analysis (BSA) approaches have been proven successful for rapidly mapping genes in plant species. However, most such methods are based on mutants and usually only one gene controlling the mutant phenotype is identified. WebMar 18, 2024 · Generally, F2 progeny is sufficient for segregant bulk construction and analysis, therefore the BSA method is often considered as rapid and efficient compared to quantitative trait locus (QTL ... coofari tools store